ALDEFLUOR KIT PDF

ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. ALDEFLUOR&#; Kit from. PRODUCT DESCRIPTION. ALDEFLUOR™ is a reagent kit that is used to identify human cells that express high levels of the enzyme aldehyde dehydrogenase. Here is a quick guide to setting up your FACS analysis with ALDEFLUOR: We’ ve used the Aldefluor kit extensively for both cell lines and for freshly dissociated .

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Swindall AF et al. Flesken-Nikitin A et al. Pierre-Louis O et al.

ALDEFLUOR™ Kit

Bone marrow should be anticoagulated with sodium heparin. Clay MR et al.

What solutions can be used to lyse erythrocytes? Review Articles Alison MR et al.

Chen D et al. Mol Kkit Ther 13 4: Todaro M et al. Cancer Res 71 Shoulars K et al. Thyroid Cancer Cells Todaro M et al. Conti L et al. Can be used with multiple cell types and species. Has supported more than peer-reviewed publications. Obermair FJ et al.

ALDEFLUOR™ Kit | STEMCELL Technologies

Tissue Dissociation and Isolation. The now well-established dependency of cancer cells on their microenvironment suggests that targeting the non-cancer-cell component of the tumour alderluor form a basis for the development of novel therapeutic approaches.

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The protocol is scalable and can be used to prepare the large number of cells required for off-the-shelf” therapies and bioengineering applications. J Cell Mol Med 15 1: Bareiss PM et al. We do not recommend use of the following or any other solution that contains a fixative, as these will render the cells nonviable: Nucl Acids Res 41 Gerber JM et al. Assay performance will not be affected.

J Aldef,uor Med 8: Yes, but full staining will take at least 3 – 4 hours. We suggest a range of concentrations from 5-fold less to fold more than the standard concentration. Importantly, we find that neutrophil-derived leukotrienes aid the colonization of distant tissues by selectively expanding the sub-pool of cancer cells that retain aldefulor tumorigenic potential. Abstract Mesenchymal stem or stromal cells MSCs have many potential therapeutic applications including therapies for cancers and tissue damages caused by apdefluor or radical cancer treatments.

Cytometry B Clin Cytom 72 4: Is it acceptable for the staining reaction to exceed 30 minutes?

Fluorescence in the test sample will additionally reflect ALDH activity. Herreros-Villanueva M et al. J Neurochem 88 1: Ovarian Cancer Cells Abelson S et al.

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For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. Storms RW et al.

Cancer Res 71 5: Rasheed Z et al. Furthermore, our data showed that the expression levels of RALDH1 in small intestinal epithelial cells correlated with the activity of vitamin A-converting enzymes in mesenteric lymph node dendritic cells, along with increased numbers of intestinal regulatory T qldefluor and a higher production of luminal IgA.

Huang EH et al. Endothelial Cells Povsic TJ et al. Vauchez K et al. Liu P et al. Buckley NE et al. Not using the assay buffer produces a proportionate loss in the assay signal, depending on the time and temperature at which the stained cells are held. Yang Y et al. Liu L et al. Piva M et al. Detection of stem and progenitor cells from normal and cancerous tissue.

Sondergaard CS et al. Liu S et al. However, different cell types may produce different results.