ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. ALDEFLUOR&#; Kit from. PRODUCT DESCRIPTION. ALDEFLUOR™ is a reagent kit that is used to identify human cells that express high levels of the enzyme aldehyde dehydrogenase. Here is a quick guide to setting up your FACS analysis with ALDEFLUOR: We’ ve used the Aldefluor kit extensively for both cell lines and for freshly dissociated .

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This transport inhibitor may not prevent efflux from other tissue types or from other species. Head Neck 32 9: This is not recommended.


Hematopoietic Stem and Progenitor Cell Research. Zhao Q et al.

The assay buffer incorporates an efflux pump inhibitor to produce optimal discrimination of the ALDHbr cells and aldeflulr maximize fluorescent signal stability. Pierre-Louis O et al. Wang L et al. Villablanca EJ et al.


Abstract Ladefluor gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. Stem Cell Rev Rep 10 1: Optimal erythrocyte lysis can be achieved with buffers containing: Brit J Cancer J Pathol 4: Bone marrow should be anticoagulated with sodium heparin.

Am Heart J 2: Cancer Res 71 Sun P et al. Explore these workflows to learn more about the other products we offer to support each research area.

Colon Cells Carpentino JE et al. Has supported more than peer-reviewed publications. J Vis Exp Stem Cell Res 5 2: Can fixed cells be used with this assay?

Mol Ther 17 It is important to ensure that reagents used for erythrocyte lysis do not contain a fixative. Identifies only viable cells with an intact cell membrane. Safety Data Sheet 4.

ALDEFLUOR™ Kit | STEMCELL Technologies

Cancer Res 69 8: For nonhematopoietic cells optimal incubation times may be different. Nakamura S et al. Cell Sourcing and Isolation.


Mol Cancer Ther 13 4: J Transl Med 8: Krishnamurthy S et al.