ALDEFLUOR KIT PDF

ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. ALDEFLUOR&#; Kit from. PRODUCT DESCRIPTION. ALDEFLUOR™ is a reagent kit that is used to identify human cells that express high levels of the enzyme aldehyde dehydrogenase. Here is a quick guide to setting up your FACS analysis with ALDEFLUOR: We’ ve used the Aldefluor kit extensively for both cell lines and for freshly dissociated .

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This transport inhibitor may not prevent efflux from other tissue types or from other species. Head Neck 32 9: This is not recommended.

ALDEFLUOR™ Kit

Hematopoietic Stem and Progenitor Cell Research. Zhao Q et al.

The assay buffer incorporates an efflux pump inhibitor to produce optimal discrimination of the ALDHbr cells and aldeflulr maximize fluorescent signal stability. Pierre-Louis O et al. Wang L et al. Villablanca EJ et al.

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Abstract Ladefluor gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. Stem Cell Rev Rep 10 1: Optimal erythrocyte lysis can be achieved with buffers containing: Brit J Cancer J Pathol 4: Bone marrow should be anticoagulated with sodium heparin.

Am Heart J 2: Cancer Res 71 Sun P et al. Explore these workflows to learn more about the other products we offer to support each research area.

Colon Cells Carpentino JE et al. Has supported more than peer-reviewed publications. J Vis Exp Stem Cell Res 5 2: Can fixed cells be used with this assay?

Mol Ther 17 It is important to ensure that reagents used for erythrocyte lysis do not contain a fixative. Identifies only viable cells with an intact cell membrane. Safety Data Sheet 4.

ALDEFLUOR™ Kit | STEMCELL Technologies

Cancer Res 69 8: For nonhematopoietic cells optimal incubation times may be different. Nakamura S et al. Cell Sourcing and Isolation.

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Mol Cancer Ther 13 4: J Transl Med 8: Krishnamurthy S et al.