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Kekik ve mercanköşk diğer yağlara göre daha yüksek antimikrobiyal aktivite gösterirken, her iki mikroorganizmaya karşı en yüksek antimikrobiyal aktiviteyi. Pazarbasi, B., Yilmaz, M., Türkiye Kosullarinda Üretimi Yapilan Spirulina platensis’ in Çesitli Ekstraktlarinin Antimikrobiyal Aktivite Kapasitesinin Belirlenmesi. antimikrobiyal aktivite, antioksidan aktivite, fenolik madde. Özet: Bu çalışmada Türkiye’nin Doğu Akdeniz Bölgesi’nden toplanan Tornabea.

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In this study, dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils were studied for their antimicrobial activity against Escherichia coli and Staphylococcus aureus by agar well diffusion method. The phenolic components are chiefly responsible for the antibacterial properties of essential oils.

However, origanum essential oil showed the highest inhibitory activity against both bacterial species tested. The obtained inhibition diameters of essential oils against E.

Browsing by Subject “Antimikrobiyal Aktivite”

A number of essential oil components has been identified as effective antibacterials, e. Numerous investigations have confirmed the antimicrobial action of essential oils. In this method obtained MIC values of E. In this study the results show that gram-negative E. The MIC values found for microplate assay was always lower that those found in turbidity measurement because of its sensitivity.

The maximum yield of dry and fresh herbage, yield and content of oil and thymol yield were obtained beginning of blooming stage. MBC is defined as concentration where Please use this identifier to cite or link to this item: Intracellular pH and ATP concentration and the release of cell constituents were measured when mustard essential oil was in contact with E.

Its effect on other parameters was very significant except dry herbage and oil yield. Essential oils, known as volatile oils, are complex mixtures of aromatic and anhimikrobiyal constituents which are obtained by such as distillation, cold pressing and maceration. Dilution methods are usually used to obtain atnimikrobiyal inhibitory concentration MIC and minimum bactericidal concentration MBC.


The MIC was determined by comparing the turbidity of the essential oil added well with antimikgobiyal turbidity of negative control added well. Results antimkrobiyal that planting space had significant effect on plant diameter and very significant effect on other measured parameters except oil content, which was not significant.

Cumin and cardamon showed the lowest antimicrobial effect against E. However, the increased demand for safe and natural food, without chemical preservatives, provokes many researchers to investigate the antimicrobial effects of natural compounds.

Although, they are GRAS, the application of essential oils is limited because of organoleptic changes in food. In the second part of this study 4 essential oils thyme, cumin, cardamon and origanum essential oils which showed antimicrobial activity against E.

In spite of modern improvements, food safety is an increasingly important public health issue. However, most studies investigating the action of whole essential oils against food spoilage organisms and food borne pathogens agree that, generally, essential oils are slightly more active against gram-positive than gram-negative bacteria.

The treatment with mustard essential oil affected the membrane integrity of bacteria and induced a decrease of the intracellular ATP concentration.

H7 and Salmonella typhi was investigated. Diffusion methods can be classified as disc diffusion and agar well diffusion methods while dilution method antimikrobiya, be classified as agar dilution and broth dilution methods. Depends on the OD measurement results, for all the tested oils, the MBC values were equivalent to the MIC values, confirming their microbicidal effects.

The obtained results are more sensitive than agar dilution method. In broth dilution studies a number of different techniques exist the most used methods are that of optical density OD turbidity measurement and the enumeration of colonies by viable count. There is some evidence that minor components have a critical part to play in antibacterial activity, possibly by producing a synergistic effect between other components. Thyme and origanum essential oils showed higher antimicrobial activity than the other essential oils.


Tests of antimicrobial activity can be classified as diffusion and dilution methods. Electronic microscopy observations showed that the cell membranes of both bacteria were apparently damaged by mustard essential oil. The obtained MIC values against E. MBC values of thyme and origanum essential a,tivite against E. But, this does not mean that gram-positive bacteria are always more susceptible.

Gram-negative bacteria are less sensitive to the antimicrobials because of the lipopolysaccharide outer membrane of this group, which restricts diffusion of hydrophobic compounds. Preservation of food materials from spoilage, mainly by microbial aktjvite, during production, storage and marketing is an important issue in the food industry.

Extensive loss of cell contents or the exit of critical molecules and ions will lead to death. Time of harvest had significant effect on yield of fresh herbage, content of oil and content of carvacrol.

Inhibitory effect of these selected essential oils were monitored during incubation period 0; 2; 4; 6; 8; 10; 12; 18, 20; 22 and 24 h and the bacterial growth was determined by measuring optical density OD at nm. Applying different methods to determine antimicrobial activity of an essential oil cause to be obtained different results.

Browsing by Subject “Antimikrobiyal Aktivite”

Show full item record. Leakage of ions and other cell contents can then occur. There are also new concerns about chemical preservatives due to increasing occurrence of many respiratory illnesses. Hydrophobicity of essential oils and their components enables them to partition in the lipids of the bacterial cell membrane, disturbing the structures and rendering them more permeable.