Keywords: Gold Nanoprism; Green Synthesis; Penicillium citrinum; Transmission , triangular gold nano plates using algal solutions of. , S. S. Shankar, A. Rai, B. Ankamwar, A. Singh, A. Ahmad and M. Sastry, “ Biological Synthesis of Triangular Gold Nanoprisms,” Nature Materials, Vol. 3, No . Synthesis of triangular gold nanoprism using lemon grass leaf extract showed UV-Visible spectra of gold nanoparticles by bio-reduction of Chloroauric acid in.
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A higher magnification image of gold nanoprism is shown in Figure C. The d-spacings calculated from FFT images of a triangular gold nanoparticle and a gold nanoprism were 2. Each experiment was repeated thrice using freshly grown culture of P.
All chemical reagents were purchased from Sigma St. Typically 2 g biomass fresh weight was dispersed in 20 ml of double distilled water pH 5. The strain was subcultured on potato dextrose agar PDA. However, there is limited information available regarding the synthesis of GNPs using fungus. In addition gold nanoprism offers several advantages in biomedical applications. Thus d-spacing of plane of gold nanoprism revealed a deviation from the standard value of fcc gold crystal 2.
Then the biomass was harvested by sieving through a plastic filter and washed several times with double distilled water to remove any traces of media components. Journal of Biomaterials and Nanobiotechnology Vol. FTIR analysis also showed that protein molecules were involved in stabilization. By conventional chemical and physical methods nanocubes , nanorods , nanowires , nanoplates  can be synthesized but that require high environmental cost.
Thus this is quite exciting for us to synthesize gold nanoprism for first time using biological approach. The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature . This microbial synthesis of GNPs could be explained by the fact that microorganisms often face metal ions or metals in their environment and thereby developed mechanism to detoxify metals by changing the redox states of metal ions and or intracellular precipitation of metals forming a basis for synthesis of nanoparticles .
Biological synthesis of triangular gold nanoprisms. – Semantic Scholar
The biologival spacing was 2. Received January 16 th; revised February 23 rd; accepted April 5 th GNPs size was found to be in the range of 10 – 40 nm with maximum population centered on 20 nm Figure 2. Due to symmetric face centered cubic fcc lattice of gold nanoparticles, the formation of anisotropic structures like cube, prism, star, branched nanoparticles require a selective capping agent during growth The physical nature of GNPs indicated that most of the nanoparticles were prism shaped.
The GNPs then resuspended in double distilled water and again centrifuged. The chemical synthesis of metal nanoprisms in large quantities using sphere-triangle shape transformation  or seeded growth  has been found to be more difficult.
FFT image also showed an elongation of the diffraction reflexes. It was quite exciting for us to find this bioprocess where mycelial biomass suspended in double distilled water in presence of chloroauric acid could produce GNPs on their surfaces.
In recent years the synthesis of stable gold nanoparticles is one of the most active areas of research in the field of nanotechnology because of their applications in nanoprusms number of areas like bio-imaging, bio-medicine, bio-sensors, bio-labels, catalysis and others .
The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature Synthesis of triangular gold nanoprism using lemon grass leaf extract showed large size of nanoprisms ranging from – nm length and truncated vertices .
UV-Visible spectra of gold nanoparticles by bio-reduction of Chloroauric acid in aqueous solution was recorded in Jasco UV-Visible spectrophotometer V operated at a resolution of 1 nm in absorption mode.
Development of strain in crystals of gold nanoprism was observed from the analysis of crystals. For FTIR spectrum analysis the GNPs were centrifuged at 10, rpm for 10 minutes to remove free proteins or other compounds present in the solution. The Scherrer ring patterns indicate the fcc gold which is nanocrystalline in nature.
Recently cetyltrimethylammonium bromide CTAB has been shown to induce the formation of gold nanorods, triangular prism and spheres through seed mediated approaches depending on iodide contamination present in CTAB which act as shape directing agent . However, bio application of large nano particles greater than 50 nm are limited due to reduced endocytosis and poor renal clearance .
Biological synthesis of triangular gold nanoprisms.
These systems are resourceful and assemble a vast assortment of materials using precursors that are readily available and abundant in the biosphere. Figure 1 B represents GNPs solution collected from mycelial surfaces following sequential sonication and filtration. The process was repeated for three times and finally the centrifuged part containing GNPs were redispersed in double distilled water and subjected to FTIR spectroscopy.
The surface plasma resonance of GNPs at nm was due to collective coherent electronic oscillation of metal nanopartticles in presence of electromagnetic field of light.